Thursday, September 26, 2019
Techniques for the Biomedical Sciences Essay Example | Topics and Well Written Essays - 1000 words
Techniques for the Biomedical Sciences - Essay Example While conducting ELISA tests mild detergents are normally used in washing out the proteins that are not bound to any particular substrate. ELISA has been widely used in pregnancy tests. Chorionic Gonadotrophin is a hormone largely produced by developing placenta during pregnancy. Peptide hormone detection is used in pregnancy tests and many other tests such as abnormally growing cell tumors most importantly the germ cell tumors. Chorionic Gonadotrophin hormone is also useful in man in the determination of cancer of the testicles. 3.A typical graph plotted will assume a sigmoid shape. This is mainly attributed to the wells from column 4 which indicate a yellow color showing positive results. However, there is increased absorption as the concentration increases. Wells that did not contain the serum showed little or no absorbance. For the wells which did not have the substrate indicated the least absorbance. This type of ELISA has been known to require the use antibody pairs which are m atching. In this case, the antibody is first coated in the pate well by an antigen molecule. The next solution known as the analyte is then applied to the well. This is then followed by addition of another antibody layer, which helps in measuring the concentration of the analyte. Capturing and detection of the analyte are possible through the use of polyclonal in sandwich ELISA is variability is allowed. In cases where the enzyme is conjugated to the detecting antibody, the process is known as direct sandwich ELISA. reduce the errors.